Biodegradation of Synthetic Reactive Orange 16 Dye in Anaerobic Reactor Conditions

Industrial use of azo dyes has been linked to severe environmental damage in the textile and apparel sectors. The breakdown products many are poisonous mutagenic, posing a threat human health environment. Hence, dye wastewater bioremediation is gaining interest. objective this study was focused on biodegradation an dye, Reactive Orange 16 (RO16), anaerobic continuous tank reactor operated with digested sludge sample. used as organic agent for dye. aim investigate fate RR16 during degradation process. Gas Chromatography-Mass Spectrophotometry (GC-MS) identify RO16 metabolites their corresponding molecular weight. As result, RO showed about 85.91% decolorization capacity, were detected pentanoic acid phenol after seven days incubation. mechanism completed bond successfully cleaved based result GC-MS, proposed pathway described. palm oil enzymes may provide new avenues biological waste treatment, especially bioremediation.